刘克海 博士 教授 博士生导师
邮箱：khliu@shou.edu.cn
研究方向:
1. 肿瘤生物治疗的纳米靶向递送技术
2. 活性多糖挖掘及其功能化产品
个人简介：
刘克海，男，1977年3月生，汉族，祖籍山东日照，中共党员，曾任食品学院院长助理、支部书记。2014年入选上海海洋大学“海燕学者”，世界中联中药药剂专业委员会委员、上海市药学会生化药学专业委员会委员，国家自然科学基金、中国博士后基金、上海市科委项目及上海市自然科学奖等评审专家。主持国家自然科学基金及各级教学、科研项目10余项；发表学术论文百余篇（ESI高被引2篇，中科院TOP 20余篇）；申请发明专利20余项；参与企业项目开发10余项，获新药证书 3项；获各级奖励 18次。2001年毕业于成都中医药大学国家理科基础科学研究和教学人才培养基地班（中药学）专业，获学士学位；2004年毕业于成都中医药大学药剂学专业，获硕士学位；2012年毕业于第二军医大学药剂学专业，获博士学位。
学术兼职：
世界中联中药药剂专业委员会委员、上海市药学会生化药学专业委员会委员
教学与课程：
《药物制剂理论与技术》《药物分析》《药物分析实验》
学术论文：
1. Song MD, Liu ZJ, Fang Z, Xu JQ, Gao XR, Zhang M*, Liu KH*. Engineered B. clausii extracellular vesicle-mediated homologous recombination impairment promotes the maintenance treatment efficacy of Olaparib in non-BRCA-mutated pancreatic cancer. Chemical Engineering Journal, 2026, 531:174116.
2. Chen F, Pan YC, Fang Z, Song MD, Zhang W, Yuan H, Gao XR, Zhang M*, Liu KH*. Camel milk extracellular vesicles-mediated co-delivery of siVEGF/sorafenib enhances the anti-hepatocellular carcinoma activity by dual-effect blocking the biological effects of VEGF, Journal of Controlled Release, 2025;385:113980.
3. Zhang M, Liu KH. Mechanisms of lipopolysaccharide-driven progression and metastasis of colorectal cancer. International Journal of Biological Macromolecules, 2025, 329(Pt 2):147856.
4. Wei SC, Zhang HT, Fang Z, Shi JW, Li BY, Liu ZJ, Song YH, Xu JQ, Yu Q, Zhang M*, Liu KH*. Copper complexes mediate the in situ production of reactive oxygen species in the nucleus, enhancing the anti-tumor efficacy of PTX. Results in Engineering, 2026, 29:109429.
5. Min Zhang; Jeevithan Elango; Xinli Guo; Hua Fan; Mingxiao Cui; Mingfu Wang; Kehai Liu*; Fe3+-Coordinated Multifunctional Elastic Nanoplatform for Effective in Vivo Gene Transfection, ACS Applied Materials & Interfaces, 2020, 12(3): 3453-3464.
6. Min Zhang; Xinli Guo; Mingfu Wang; Kehai Liu*; Tumor microenvironment-induced structure changing drug/gene delivery system for overcoming delivery-associated challenges, Journal of Controlled Release, 2020, 323: 203-224.
7. Yang, Deyu; Fang, Zhou; Kang, Rui; Liu, Kehai*; Composite electrospun scaffold containing decellularized amniotic matrix for pelvic organ prolapse, Materials and Design, 2021, 210: 0-110106.
8. Mengdi Song; Mingxiao Cui; Zhou Fang; Kehai Liu*; Advanced research on extracellular vesicles based oral drug delivery systems, Journal of Controlled Release , 2022, 351: 560-572.
9. Cui MX, Zhang M, Liu KH*. Colon-targeted drug delivery of polysaccharide-based nanocarriers for synergistic treatment of inflammatory bowel disease: A review. Carbohydrate polymers, 2021, 272: 118530.
10. Fang Z, Liu K*. Plant-derived extracellular vesicles as oral drug delivery carriers. J Control Release. 2022, 350:389-400.
11. Huiting Chen; Zhou Fang; Mengdi Song; Kehai Liu*; Mitochondrial targeted hierarchical drug delivery system based on HA-modified liposomes for cancer therapy, European journal of medicinal chemistry , 2022, 241: 114648-114648.
12. Song MD, Cui MX, Liu KH*. Therapeutic strategies to overcome cisplatin resistance in ovarian cancer. Eur J Med Chem, 2022, 232: 114205-114205. （ESI高被引论文）
13. J. Xu, M. Song, Z. Fang, L. Zheng, X. Huang, K. Liu*, Applications and challenges of ultra-small particle size nanoparticles in tumor therapy, J. Contr. Release, 2022, 353: 699-712.（ESI高被引论文）
14. Ruimei Zhou, Mingxiao Cui, Yu Wang, Min Zhang, Feifei Li, Kehai Liu*. Isolation, structure identification and anti-inflammatory activity of a polysaccharide from Phragmites rhizoma. International Journal of Biological Macromolecules, 2020,161,810-817.
15. Cui MX, Zhang M, Wu JW, Han P, Lv M, Dong L, Liu KH*. Marine polysaccharides from Gelidium pacificum Okamura and Cereus sinensis reveal prebiotic functions. International Journal of Biological Macromolecules, 2020, 164: 4381-4390.
16. Mingxiao Cui, Junwen Wu, Shuyue Wang, Hongmei Shu, Min Zhang, Kehai Liu*, Kewu Liu. Characterization and anti-inflammatory effects of sulfated polysaccharide from the red seaweed Gelidium pacificum Okamura. International Journal of Biological Macromolecules. 2019, 129:377–385.
17. Kehai Liu, Xiaoyu Wang, Wei Fan, Qing Zhu, Jingya Yang, Jing Gao, Shen Gao. Degradable polyethylenimine derivate coupled to a bifunctional peptide R13 as a new gene delivery vector. International Journal of Nanomedicine, 2012, 7:1149-1162.
18. Kehai Liu, Yanqin Xu, Xichang Wang. Microencapsulation of sweet orange oil terpeneless using the orifice method. Journal of Food Engineering, 2012, 110:390-394.
19. Chengchu Liu, Qing Zhu, Wenhui Wu, Xiaolin Xu, Xiaoyu Wang, Shen Gao Kehai Liu*. Degradable copolymer based on amphiphilic N-octyl-N-quatenary chitosan and low molecular weight polyethylenimine for gene delivery. International Journal of Nanomedicine, 2012, 7:5339-5350.
20. Hui Lv, Qing Zhu, Kewu Liu, Manman Zhu, Wenfang Zhao, Yuan Mao, Kehai Liu*. Coupling of a bifunctional peptide R13 to OTMCS-PEI copolymer as gene vector increases transfection efficiency and tumor targeting. International Journal of Nanomedicine, 2014, 9:1-12.
21. Cui MX, Wang Y, Elango J, Wu JW, Liu KH*, Jin YZ*. Cereus sinensis polysaccharide alleviates antibiotic-associated diarrhea based on modulating the gut microbiota in C57BL/6 mice. Frontiers in Nutrition, 2021, 8: 751992.
22. Cui MX, Zhou RM, Wang Y, Zhang M, Liu KH*, Ma CC*. Beneficial effects of sulfated polysaccharides from the red seaweed Gelidium pacificum Okamura on mice with antibiotic-associated diarrhea. Food & Function, 2020, 11(5): 4625-4637.
23. Cui M, Fang Z, Song M, Zhou T, Wang Y, Liu K*. Phragmites rhizomapolysaccharide-based nanocarriers for synergistic treatment of ulcerative colitis. Int J Biol Macromol. 2022 Nov 1;220:22-32.
24. Junwen Wu , Xuefei Zhou , Min Zhang , Yun Yao , Juanjuan Han and Kehai Liu*. Cereus Sinensis Polysaccharide and Its Immunomodulatory Properties in Human Monocytic Cells. Marine Drugs. 2017,15(5):1-13.
25. Xu J, Song M, Fang Z, Zheng L, Wang J, Liu K*. A chemoimmunotherapy-based strategy to enhance tumor therapy by cross-linking a novel size-variable nanocluster via a bifunctional peptide[J]. Materials & Design, 2023, 233: 112232.
26. Fang Z, Song MD, Lai KQ, Cui MX, Yin MY, Liu KH*. Kiwi-derived extracellular vesicles for oral delivery of sorafenib. Eur J Pharm Sci 191 (2023) 106604
27. Song MD, Fang Z, Wang J*, Liu KH*. 2023. A nano-targeted co-delivery system based on gene regulation and molecular blocking strategy for synergistic enhancement of platinum chemotherapy sensitivity in ovarian cancer. Int J Pharmaceut, 641: 123022.
28. Zhou Fang, Min Zhang, Rui Kang, Mingxiao Cui, Mengdi Song, Kehai Liu*. A cancer cell membrane coated nanoparticles-based gene delivery system for enhancing cancer therapy. International Journal of Pharmaceutics, 629 (2022) 122415.
29. Zhang M, Fang Z, Zhang H, Cui M, Wang M, Liu K*. Reversing tumor immunosuppressive microenvironment via targeting codelivery of CpG ODNs/PD-L1 peptide antagonists to enhance the immune checkpoint blockade-based anti-tumor effect. Eur J Pharm Sci. 2022 Jan 1;168:106044.
30. Zhang M, Fang Z, Cui M, Liu K*. Multifunctional metal complex-based gene delivery for tumour immune checkpoint blockade combination therapy. J Drug Target. 2022 Aug;30(7):753-766.
31. Kang R, Song M, Fang Z, Liu K*. Nano-composite hydrogels of Cu-Apa micelles for anti-vasculogenic mimicry. J Drug Target. 2022 Aug 31:1-13.
32. Kehai Liu, Qiulin Chen, Yanjun Liu, Xiaoyan Zhou, Xichang Wang. Isolation and biological activities of decanal, linalool, valencene, octanal from sweet orange oil. Journal of Food Science. 2012, 77(11):C1156-C1161.
33. Min Zhang, Jing Hu, YingZou, Junwen Wu, YunYao, Hua Fan, Kehai Liu*, Jun,Wang,Shen Gao. Modification of degradable nonviral delivery vehicle with a novel bifunctional peptide to enhance transfection in vivo. Nanomedicine. 2018,13(1):9-24.
34. Li F, Liu K, Liu K*. Chemical characterization of a new sulfated polysaccharide from Gracilaria chouae and its activation effects on RAW264.7 macrophages. J Zhejiang Univ Sci B. 2022 Jan 15;23(1):84-88.
研究项目：
1. 国家自然科学基金项目—工程化Bacillus clausii胞外囊泡口服递送PD-L1自阻断质粒协同抗胰腺癌作用研究；
2. 国家自然科学基金项目—双功能肽tLyP-1-NLS修饰的DNA纳米胶束的构建及其抗肿瘤作用研究;
3. 国家自然科学基金项目—壳聚糖季铵盐连接LMW-PEI作为骨架的靶向DNA纳米复合物的构建及其抗肿瘤作用研究；
4. 国家自然科学基金项目—基于基因调控与分子阻断策略的融合型共递送系统协同增强卵巢癌铂化疗敏感性研究;
5. 上海市科委“科技创新行动计划”国际学术合作项目—PEI-K13/p53纳米复合物逆转卵巢癌腹腔转移瘤的化疗耐药性研究;
6. 上海市教委科研创新项目—三功能肽R18修饰的DNA纳米胶束的构建及其细胞核转运机制;
7. 上海市教委高校选拔培养优秀青年教师科研专项基金—外用抗灰指甲给药系统探讨；
8. 农业部中德农业科技合作项目—基于结构特征研究生物多糖活性作用的技术；
9. “十五”国家中医药科技攻关项目—贯众饮片炮制规范化研究；
10. 国家“十一五”科技支撑计划—大宗低值鱼加工新产品与超低温急冻装备开发及产业化示范。
奖励荣誉：
曾获上海市教学成果一等奖、国家海洋局海洋科学技术奖二等奖、浦东新区科技奖三等奖及上海海洋大学育才奖、教学成果二等奖、科学成果三等奖、优秀共产党员、“好课堂”等。
课题组成员介绍：
张敏，女，中共党员，上海海洋大学生物制药专业讲师。2022年入选“博新计划”、上海市“超级博士后”项目，上海市药学会生化药物专业委员会青年委员。主持及参与中国博士后科学基金面上项目、国家自然科学基金等多项科研项目；发表学术论文20余篇；申请多项发明专利。2021年毕业于上海海洋大学，获博士学位。2021年至2023年于中国科学院上海药物研究所开展博士后研究工作。主要研究方向：功能因子的靶向递送与可控释放。